1993 – 2008  Senior research associate.

• Biomedical Research Unit, Jessop Wing, Sheffield, in the laboratory of Professor TC Li. Investigations into the underlying causes of unexplained recurrent miscarriage and the failure of embryo implantation after in vitro fertilisation.
• External lecturer (Ethics and Assisted Reproductive Technologies), Sheffield Hallam University.

1991 – Locum cytogeneticist

• Centre for Human Genetics, Sheffield.

1983-87 – Research associate

• Department of Clinical Genetics, University of Birminghamin the laboratory of Dr Tessa Webb. Investigation into the effect of Lyonisation on the phenotypic expression of the Fragile X Syndrome in female heterozygotes.

1981 – Locum cytogeneticist

• Regional Cytogenetics Department, East Birmingham Hospital.

1970-1974 – Research associate

• Department of Haematological Medicine, University of Cambridge. Organised a cytogenetics service for the United Cambridge Hospitals and undertook research into the relationship between viruses, chromosomes and leukaemia with Dr’s A. Karpas and J. Cawley. Analysed the chromosomes of the first hybridoma produced by Dr’s Milstein and Cotton.

1968-1970 – Research assistant

• Department of Human Anatomy, University of Newcastle upon Tyne. Worked in the laboratory of Dr John Gray, an expert in intersexuality and head of the cytogenetics service for the Royal Victoria Infirmary Hospital, and surrounding hospitals Newcastle-upon-Tyne. Prepared and analysed chromosomes from peripheral blood and bone marrow in order to identify syndromes due to chromosome abnormalities. These fell into 4 main groups (i) birth defects (ii) abnormal sexual development (iii) infertility (iv) blood disorders e.g. leukaemia.

 

Endometrial cells in culture

 

SELECTED PUBLICATIONS

Mariee N, Tuckerman E, Ali A, Li W, Laird S, Li TC.(2012) The observer and cycle-to-cycle variability in the measurement of uterine natural killer cells by immunohistochemistry. J Reprod Immunol. Sep;95(1-2):93-100

Mariee NG, Tuckerman E, Laird S, Li TC. (2012) The correlation of autoantibodies and uNK cells in women with reproductive failure. J Reprod Immunol. Sep;95(1-2):59-66.

Udayashankar R, Baker D, Tuckerman E, Laird S, Li TC, Moore HD (2011) Characterization of invasive trophoblasts generated from human embryonic stem cells. Hum Reprod. Feb;26(2):398-406.

Tuckerman E, Mariee N, Prakash A, LI TC, laird S. (2010) Uterine natural killer cells in peri-implantation endometrium from women with repeated implantation failure after IVF. J Reprod Immunol. Dec;87(1-2):60-6.

Prakash A, Tuckerman E, Laird S, Ola B, Li TC, Ledger WL. (2008) A preliminary study comparing the endometrial expression of inhibin, activin, and follistatin, in women with a history of implantation failure after IVF treatment and a control group. BJOG 115, 532-6 : discussion 536-7.

Cocksedge KA, Saravelos SH, Wang Q, Tuckerman E, Laird SM, Li TC (2008) Does free androgen index predict subsequent pregnancy outcome in women with recurrent miscarriage? Hum Reprod 23, 797-802. Hum._Reprod.-2008-Cocksedge-797-802[1]

Tuckerman E., Laird S.M., Prakash A. and Li T.C. (2007) Prognostic value of the measurement of uterine natural killer (uNK) cells in the endometrium of women with recurrent miscarriage. Hum Reprod 22, 2208-13. Hum._Reprod.-2007-Tuckerman-2208-13[1]

Metwally M, Tuckerman EM, Laird SM, Ledger WL, Li TC. (2007) Impact of high body mass index on endometrial morphology and function in the peri-implantation period in women with recurrent miscarriage. Reprod. Biomed Online 14, 328-34.

Monkkonen KS, Aflatoonian R, Lee KF, Yeung WS, Tsao SW, Laitinen JT, Tuckerman EM, Li TC, Fazeli A. (2007) Location and variable expression of G(alpha)i2 in human endometrium and fallopian tubes. Hum. Reprod. 22, 1224-30. Hum._Reprod.-2007-Mönkkönen-1224-30[1]

Aflatoonian R, Tuckerman E, Elliot SL, Bruce C, Aflatoonian A, Li TC, Fazeli A. (2007) Menstrual cycle-dependent changes of Toll-like receptors in endometrium. Hum Reprod 22, 586-93. Hum._Reprod.-2006-Aflatoonian-586-93[1]

Tuckerman EM, Laird SM, Prakash A, Li TC (2006) Expression of integrins in the endometrium of women with recurrent miscarriage. Fetil Steril 86, 755-7.

Prakash A, Li TC, Tuckerman E, Laird S, Wells M, Ledger WL. (2006) A study of luteal phase expression of inhibin, activin, and follistatin subunits in the endometrium of women with recurrent miscarriage. Fertil. Steril. 86, 1723-30.

Laird SM, Tuckerman EM, Li TC (2006) Cytokine expression in the endometrium of women with implantation failure and recurrent miscarriage. Reprod. Biomed. Online 13, 13-23.

Prakash A, Laird S, Tuckerman E, Li TC, Ledger WL. (2005) Inhibin A and activin A may be used to predict pregnancy outcome in women with recurrent miscarriage. Fertil. Steril. 83, 1758-63.

Lijawi S, Li TC, Tuckerman EM, Blakemore AL, Laird SM (2004) Expression of interleukin-11 receptor alpha and interleukin-11 protein in the endometrium of normal fertile women and women with recurrent miscarriage. J. Reprod. Immunol. 64, 145-55.

Tuckerman E, Laird SM, Stewart R, Wells M, Li TC (2004) Markers of endometrial function in women with unexplained recurrent pregnancy loss: a comparison between morphologically normal and retarded endometrium. Hum. Reprod. 19, 196-205. Hum._Reprod.-2004-Tuckerman-196-205[1]

Quinton ND, Laird SM, Tuckerman EM, Cork BA, Li TC. (2003) Expression of leptin receptor isoforms in vitro: lack of effects of leptin on endometrial cytokine and MMP production. (2003) Am. J. Reprod. Immunol. 50, 224-31.

Laird SM, Tuckerman EM, Cork BA, Linjawi S, Blakemore AI Li TC (2003) A review of immune cells and molecules in women with recurrent miscarriage. Hum. Reprod. Update 9, 163-74. Hum._Reprod._Update-2003-Laird-163-74[1]

Li TC, Mackris M, Tomsu M, Tuckerman E, Laird S (2002) Recurrent miscarriage: aetiology, management and prognosis. Hum. Reprod. Update 8, 463-81. Hum._Reprod._Update-2002-Li-463-81[1]

Cork BA, Tuckerman EM, Li TC, Laird SM (2002) Expression of interleukin (IL)-11 receptor by the human endometrium in vivo and effects of IL-11, IL-6 and LIF on the production of MMP and cytokines in vitro. Mol. Hum. Reprod. 8, 841-8. Mol._Hum._Reprod.-2002-Cork-841-8[1]

Li TC, Tuckerman EM, Laird SM (2002) Endometrial factors in recurrent miscarriage Hum. Reprod. Update 8, 43-52. Hum._Reprod._Update-2002-Li-43-52[1]

Page M, Tuckerman EM, Li TC, Laird SM (2002) Expression of nuclear factor kappa B components in human endometrium. J. Reprod. Immunol. 54, 1-13.

Li TC, Spuijbroek MD, Tuckerman EM, Anstie B, Loxley M (2000) Endocrinological and endometrial factors in recurrent miscarriage. BJOG 107, 1471-9.

Li TC, Ding SH, Anstie B, Tuckerman E, Wood K, Laird S (2001) Use of human menopausal gonadotrophins in the treatment of endometrial defects associated with recurrent miscarriage: preliminary report. Fertil. Steril. 75, 434-7.

Tuckerman EM, Okon MA, Li TC, Laird SM (2000) Do androgens have a direct effect on endometrial function? An in vitro study. Fertil. Steril. 74, 771-9.

Laird SM, Tuckerman EM, Cork BA, Li TC (2000) Expression of nuclear factor kappa B in human endometrium; role in the control of interleukin 6 and leukaemia inhibitory factor production. Mol. Hum. Reprod. 6, 34-40. Mol._Hum._Reprod.-2000-Laird-34-40[1]

Okon MA, Laird SM, Tuckerman EM, Li TC (1998) Serum androgen levels in women who have recurrent miscarriages and their correlation with markers of endometrial function. Fertil. Steril. 69, 682-90.

Laird SM, Tuckerman EM, Dalton CF, Dunphy BC, Li TC, Zang X (1997) The production of leukaemia inhibitory factor by human endometrium: presence in uterine flushings and production by cells in culture. Hum. Reprod. 12, 569-74. Hum._Reprod.-1997-Laird-569-74[1]

Laird SM, Tuckerman EM, Saravelos H, LI TC (1996) The production of tumour necrosis factor alpha (TNF-alpha) by human endometrial cells in culture. Hum. Reprod. 12, 569-74. Hum._Reprod.-1996-Laird-1318-23[1]

Laird SM, Hill CJ, Warren MA, Tuckerman EM, Li TC (1995) The production of placental protein 14 by human tubal epithelial cells in culture. Hum. Reprod. 10, 1346-51.

Laird SM, Tuckerman E, LI TC, Bolton AE (1994) Stimulation of human endometrial epithelial cell interleukin 6 production by interleukin 1 and placental protein 14. Hum. Reprod. 9, 1339-43.

Tuckerman E, Webb T (1989) The inactivation of the fragile X chromosome in female carriers of the Martin Bell syndrome as studied by two different methods. Clin Genet. 36, 25-30.

Tuckerman E, Webb T, Thake A (1986) Replication status of fragile X (q27.3) in 13 female heterozygotes. J. Med. Genet. 23, 407-10. jmedgene00091-0023[1]

Tuckerman E, Webb T, Bundey SE (1985) Frequency and replication staus of the fragile X, fra(X)(q27-28) in a pair on monozygotic twins of markedly differing intelligence. J. Med. Genet. 22, 85-91. jmedgene00094-0005[1]

Fragile X  Culture after BuDr incorporation to show the early replicating active X, which has two small chromosome fragments hanging off the end of the long arm and is therefore fragile (arrowed) and the normal pale stained late replicating inactive X.

 

Karpas A, McGeoch J, Tuckerman E, Cawley J (1974) Letter: Viral-coded information in human rhabdomyosarcoma cells. Lancet 2, 1330-1.

Karpas A, Tuckerman E. (1974) Transformation of human fibroblasts with DNA of cultured rhabdomyosarcoma cells. Lancet 8, 1138-41.

Karpas A, Cawley J, Tuckerman E (1972) Cytological, cytogenetic, and ultrastructural studies of rat cell lines carrying avian and murine sarcoma genomes. Krebsforsch. Klin. Onkol. Cancer. Res. Clin. Oncol. 78, 51-64.

Karpas A, Cawley J, Tuckerman E, Flemans R, Hayhoe FG (1971) Cytochemistry, cytogenetics, and ultrastructure of hamster tumour cells carrying mouse sarcoma viral genome (HT-1 cells). Br. J. Cancer 25, 779-88. brjcancer00361-0191[1]

Tuckerman E, Gray JE, Salmon J (1970) Familial transmission of an unusual variant of a group C chromosome. Ann. Genet. 13, 183-6.